Eksplorasi, Isolasi, dan Identifikasi Jamur Entomopatogen Asal Rizosfer Tanaman Padi
DOI:
https://doi.org/10.53863/agronu.v5i02.2165Keywords:
biological agents, organic carbon level, entomopathogen, insect bait method, rhizosferAbstract
Rice pest management in the field predominantly depends on the application of chemical pesticides. However, the use of entomopathogenic fungi represents an alternative strategy within Integrated Pest Management (IPM) programs. This study aimed to examine the relationship between soil organic matter content and soil pH with the presence of entomopathogenic fungi, as well as to identify these fungi morphologically from the rhizosphere of rice plants using the bait insect method. The research was conducted from July to October 2025 and involved several stages, including soil sampling and analysis, isolation and collection of entomopathogenic fungi, morphological identification, and data analysis. Soil samples were collected from the regencies of Blora, Jepara, Kebumen, and Semarang. The results indicated that the soil samples were categorized into low, medium, and high organic carbon levels, with soil pH ranging from 6.50 to 7.99. Morphological identification revealed that Aspergillus sp. was present in isolates coded BB1, BB2, KK1, KK3, SA2, and SA3, showing both microscopic and macroscopic characteristics consistent with established references. The detection of entomopathogenic fungi in the rice rhizosphere highlights their ecological role in maintaining agroecosystem balance and their potential to naturally suppress pest populations. Nevertheless, molecular identification is required to accurately determine the fungal species prior to conducting further evaluations, such as pathogenicity, virulence, and efficacy tests against target pests, before large-scale application can be considered
References
Elan Ismail, Mohamad Lihawa, Indriati Husain, R. I. (2016). Uji Efektivitas jamur Entomopatogen Beauveria bassiana untuk Mengendalikan Spodoptera frugiperda pada Tanaman Jagung. Kanal, 3(2), 1–23.
Gary E. Harman, C. P. K. (1998). Trichoderma And Gliocladium. Volume 1 Basic Biology, Taxonomy and Genetics.
Ginting S, Santoso T, H. I. (2024). Patogenisitas beberapa isolat cendawan entomopatogen terhadap Coptotermes curvignathus Holmgren dan Schedorhinotermes javanicus Kemmer. Jurnal Agroekoteknologi Tropika. 2(1): 20‒33. Agroekoteknologi Tropika. 2(1): 20‒33., 2(1), 61–64.
Ginting, S., Pamekas, T., & Neildi, Z. (2024). Exploration, Isolation dan Identification Entomopathogenic fungi from Rhizosphere of Corn Plants in Bengkulu using Baiting Insect Methods. Jurnal Agrikultura, 35(2), 308–320.
Hare, J. (2016). Sabouraud Agar for Fungal Growth. Laboratory Protocols in Fungal Biology. https://doi.org/10.1007/978-1-4614-2356-0_15
Humber, R. A. (2012). Identification of entomopathogenic fungi. In Manual of Techniques in Invertebrate Pathology (Issue December 2012). https://doi.org/10.1016/B978-0-12-386899-2.00006-3
Jeon, C. S., Kim, G. H., Son, K. I., Hur, J. S., Jeon, K. S., Yoon, J. H., & Koh, Y. J. (2013). Root rot of balloon flower (Platycodon grandiflorum) caused by Fusarium solani and Fusarium oxysporum. Plant Pathology Journal, 29(4), 440–445. https://doi.org/10.5423/PPJ.NT.07.2013.0073
Lubis, A. A. N., Anwar, R., Soekarno, B. P., Istiaji, B., Sartiami, D., Irmansyah, & Herawati, D. (2020). Serangan ulat grayak jagung (Spodoptera frugiperda) pada tanaman jagung di Desa Petir, Kecamatan Daramaga, Kabupatem Bogor dan potensi pengendaliannya menggunakan Metarizhium Rileyi. Jurnal Pusat Inovasi Masyarkat, 2(6), 931–939.
Maniania, N. K., Jaronski, S. T., & Plains, N. (2007). Soil ecology of the entomopathogenic Ascomycetes : A critical examination of what we ( think ) we know (Vol. 661, Issue 2).
Mawarni, N. I. I., Erdiansyah, I., & Wardana, R. (2021). Isolasi Cendawan Aspergillus sp. pada Tanaman Padi Organik. Agriprima : Journal of Applied Agricultural Sciences, 5(1), 68–74. https://doi.org/10.25047/agriprima.v5i1.363
Meyling, N. V., & Eilenberg, J. (2007). Ecology of the entomopathogenic fungi Beauveria bassiana and Metarhizium anisopliae in temperate agroecosystems: Potential for conservation biological control. Biological Control, 43(2), 145–155. https://doi.org/10.1016/j.biocontrol.2007.07.007
Mohammed, A. A., Kadhim, J. H., & Kamaluddin, Z. N. A. (2018). Selection of highly virulent entomopathogenic fungal isolates to control the greenhouse aphid species in Iraq. Egyptian Journal of Biological Pest Control, 28(1). https://doi.org/10.1186/s41938-018-0079-3
Nelly, N., Syahrawati, M., Hamid, H., Habazar, T., & Gusnia, D. N. (2019). Diversity and characterization of entomopathogenic fungi from rhizosphere of maize plants as potential biological control agents. Biodiversitas, 20(5), 1435–1441. https://doi.org/10.13057/biodiv/d200536
Pangesti, N., Pineda, A., Pieterse, C. M. J., Dicke, M., & Loon, J. J. A. (2013). Two-way plant-mediated interactions between root-associated microbes and insects: From ecology to mechanisms. Frontiers in Plant Science, 4(OCT), 1–11. https://doi.org/10.3389/fpls.2013.00414
Quesada-Moraga, E., Navas-Cortés, J. A., Maranhao, E. A. A., Ortiz-Urquiza, A., & Santiago-Álvarez, C. (2007). Factors affecting the occurrence and distribution of entomopathogenic fungi in natural and cultivated soils. Mycological Research, 111(8), 947–966. https://doi.org/10.1016/j.mycres.2007.06.006
Safitri, A., Herlinda, S., & Setiawan, A. (2018). Entomopathogenic fungi of soils of freshwater swamps, tidal lowlands, peatlands, and highlands of south sumatra, Indonesia. Biodiversitas, 19(6), 2365–2373. https://doi.org/10.13057/biodiv/d190647
Salasa, T., Tarore, D., & Rimbing, J. (2024). Patogenisitas Jamur Entomopatogen Metarhizium Anisopliae Metch. Terhadap Larva Spodoptera Litura F. Pada Tanaman Kubis (Brassicae Oleracea L.) Di Laboratorium. Agri-Sosioekonomi, 20(2), 661–668. https://doi.org/10.35791/agrsosek.v20i2.57233
Sari, R., Maryam, & R., & A.Yusamah. (2023). Vol. 12, No. 1, Tahun 2023. Penentuan C-Organik Pada Tanah Untuk Meningkatkan Produktivitas Tanaman Dan Berkelanjutan Umur Tanaman Dengan Metoda Spektrofotometri UV VIS, 12(1), 11–19. dewi.a@unidha.ac.id
Sharma, L., Oliveira, I., Torres, L., & Marques, G. (2018). Entomopathogenic fungi in Portuguese vineyards soils: Suggesting a ‘Galleria-Tenebrio-bait method’ as bait-insects galleria and tenebrio significantly underestimate the respective recoveries of metarhizium (robertsii) and beauveria (bassiana). MycoKeys, 38, 1–23. https://doi.org/10.3897/mycokeys.38.26970
Sosa-Gómez, D. R., Delpin, K. E., Moscardi, F., & Farias, J. R. B. (2001). Ocorrência natural de fungos entomopatogênicos Metarhizium, Beauveria e Paecilomyces na cultura da soja sob plantio direto e convencional. Neotropical Entomology, 30(3), 407–410. https://doi.org/10.1590/S1519-566X2001000300011
Sudarjat, S., Hersanti, H., & Nurazizah, R. (2023). Aplikasi Jamur Entomopatogen Lecanicillium lecanii pada Berbagai Kerapatan Konidia dan Frekuensi Aplikasi terhadap Hama Kutukebul (Bemisia tabaci) pada Tanaman Tomat. Agrikultura, 34(2), 274. https://doi.org/10.24198/agrikultura.v34i2.47658
Sudiarta, I.P.,*, R. D. Syahbana, K. A. Yuliadhi, I.P.W. Suputra, I. W. D. G. (2024). Eksplorasi Jamur Entomopatogen, dari Beberapa Rizosfer Tanah, Dengan Insect Bait Method Exploration of Entomopathogenic Fungi, from Several Soil Rhizospheres, Using the Insect Bait Method. 17(1), 105–117.
TRIZELIA, & , NELDI ARMON, H. J. (2015). Keanekaragaman cendawan entomopatogen pada rizosfer berbagai tanaman sayuran. 1(Nur 2005), 998–1004. https://doi.org/10.13057/psnmbi/m010307
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2026 Chaieydha Noer Afiefah, Nani Kitti Sihaloho, Yudhan Saylendra Anugratama, Naili Arzaqul Barokah
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution-ShareAlike 4.0 International License that allows others to share the work with an acknowledgment of the work’s authorship and initial publication in this journal
